1/15/2024 0 Comments Taurine bull spermFollicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T) and estradiol (E) radioimmunoassay kits were purchased from Beijing Chemclin Biotech Co., Ltd. Taurine and β-alanine(β-Ala) were purchased from Sigma (USA). The study protocol was approved by our Ethical Committee and conducted in compliance to the Helsinki Declaration. Rats were given different treatment as female rats respectively. 30 male rats aged 10 weeks or 72 weeks were dived into 3 groups respectively, 10 in each group. Rats in the control group were given tap water, the β-alanine group was given water containing 1%β-alanine, and the taurine group was given water containing 1% taurine. Female rats were given different water from the day of confinement which was defined as the first day of the newborn rats. 15 pregnant rats with the similar expected confinement date were divided into three groups randomly, five in each group. Impregnation was determined if sperm were detected on the second day. After acclimatizing for 1 week, all rats were used in the experiment.įemale rats were put together with male rats in cages after the oestrus of female rats were determined by vaginal smear. They were maintained in controlled light (14h-light, 10h-dark) and temperature (22☒☌), and were given free access to rat chow and water. Male rats were 10 (adult) and 72 (aged) weeks old, female rats were 10 weeks old (about 200 g). The correlation between the decline of taurine content and aged male reproduction was also evaluated in the present study.Īll Wistar rats were obtained from the central animal house of the Chinese Medical University. However, a number of studies have reported that the concentration of taurine is obviously decreased in serum and some tissues of aged animals. It is well known that aging results in a significant decline in male reproduction. The primary aim of the present study was to further investigate the effect of taurine on male reproduction in rats. Despite its importance, the effect of taurine on the male reproduction is still unclear. Our laboratory had previously reported that taurine can stimulate testosterone secretion in vivo and in vitro. Taurine may act as an antioxidant, capacitating agent, membrane-stabilized factor and motility factor of sperm. In addition, taurine has been identified as the major free amino acid of sperm cells and seminal fluid. It has also been reported that taurine can be biosynthesized by male reproductive organs. In the male reproductive system, taurine has been detected in Leydig cells, vascular endothelial cells, and some other interstitial cells of testis and epithelial cells of efferent ducts in rats. However, several physiological functions of taurine have been demonstrated, such as osmoregulation, calcium modulation, membrane stabilization, antioxidation, radioprotection, energy storage, xenobiotic conjugation, isethionic acid and anion balance et al.Īs a semi-essential amino acid, taurine is rich in many tissues, and can be biosynthesized by many tissues, such as the central nervous system, liver, kidney, retina and mammary gland. Taurine is not involved in protein synthesis or in metabolic pathway. Taurine, 2-aminoethane sulphonic acid, is one of the most abundant low-molecular-weight organic constituents in human and many animals. The results imply that taurine plays important roles in male reproduction especially in aged animals. The present study demonstrated that a taurine supplement could stimulate the secretion of LH and T, increase the levels of testicular marker enzymes, elevate testicular antioxidation and improve sperm quality. The numbers and motility of spermatozoa, the rate of live spermatozoa were significantly increased by taurine supplement in aged rats. The motility of spermatozoa was obviously increased by taurine supplement in adult rats. The levels of SOD, ACP, LDH, SDH, NOS, NO and GSH were significantly elevated by taurine administration in aged rats, but the levels of AST and ALT were significantly decreased. The levels of SOD, ACP, SDH and NOS were obviously increased by taurine administration in adult rats, but the levels of AKP, AST, ALT and NO were significantly decreased. The levels of T and LH were obviously increased by taurine supplementation in rats of different ages, and the level of E was also significantly elevated in baby rats. The effects of taurine on reproductive hormones, testis marker enzymes, antioxidative ability and sperm quality were investigated. Taurine and β-alanine (taurine transport inhibitor) were offered in water to male rats of different ages. But the effect of taurine on male reproduction is poorly understood. It has been demonstrated that taurine is one of the most abundant free amino acids in the male reproductive system, and can be biosynthesized by male reproductive organs.
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